MODELING OF GLYCOPROTEIN PRODUCTION BY CHINESE HAMSTER OVARY CELLS FOR PROCESS MONITORING AND CONTROL

Substantial improvements of animal cell culture process performance can be achieved by model-based methods of process optimization and control. Here, a structured mathematical model describing the dynamics of Chinese hamster ovary (CHO) cell growth and recombinant glycoprotein production has been developed. It covers the main aspects of cell metabolism as well as aspects linked to cell cycle regulation. Model predictions fit well to experimental data obtained from fed-batch cultures and allow for insight into the complex behaviour of CHO cells in culture. Model-based improvement of feeding trajectories is shown. Further applications for process monitoring and control are discussed.