A kind of recombination glutamate producing bacterium strain and the preparation method and application thereof

The invention discloses a kind of methods of preparation and reorganization glutamate producing bacterium strain, this method comprises: inactivating the gene pitR in glutamate producing bacterium strain and making non-PTS glucose transport systems gene overexpression, the non-PTS glucose transport systems gene includes gene iolT1 and/or iolT2；And/or, it inactivates PTS glucose transport systems gene and makes glucokinase gene overexpression, the PTS glucose transport systems gene is selected from least one of gene ptsI, ptsH or ptsG, and the glucokinase gene includes gene glk and/or ppgK.The application in glutamic acid is produced the invention also discloses recombination glutamate producing bacterium strain prepared by the above method and its in fermentation.By above technical scheme, that present invention obtains inversion rate of glucose is big, glutamic acid yield is high recombination glutamate producing bacterium strain, that is, recombination glutamate producing bacterium strain production performance with higher of the invention.