Normal variations in the morphology of auditory brainstem response (ABR) waveforms: a study in wistar rats

Abstract Auditory brainstem evoked responses (ABR) have been used for decades to assess auditory function. Surprisingly, despite the fact that rats are one of the most widely used experimental models in hearing, there have been no studies that have characterized in detail the normal morphological variations that occur in ABR waves. Therefore, the goal of this study was to characterize the patterns of ABR waves in rats to establish baseline criteria that could be used to identify abnormalities. Rats were stimulated with pure tone sounds at different frequencies and ABR waves were classified based on morphology. The most definitive finding was that, unlike what is observed in human ABRs, wave II of the rat ABR was the most prominent. Additionally, wave III was the smallest and, in many cases, was not apparent at low frequencies. Wave III was frequently involved in the formation of complexes, often appearing as a small wave or adjoining primarily wave IV. Complexes were common at low and medium frequencies and rare at high frequencies. These results indicate that knowledge of the different wave patterns in normal rats is fundamental to understanding how the wave morphology changes in pathological conditions that could lead to hearing impairment.