Interspecific Polymorphism of Gene lipL32 Restriction Profiles of Pathogenic Leptospires

We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three leprospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profi les of the studied gene were similar at both the intraspecifi c and serogroup levels; variability was revealed only at the interspecifi c level, which can be explained by relatively low variability of lipL32 gene.