Microscope-type laser speckle contrast imaging for in vivo assessment of microcirculation

Laser speckle contrast imaging (LSCI) can be used to observe dynamic changes in the tissue microcirculation in vivo according to the dynamic interaction between red blood cells and coherent light. In this study, a dual-wavelength LSCI system based on a microscope was developed for in vivo observation of the microvascular pattern and measurement of the blood flow change in the animal model. Additionally, based on the dual-wavelength setup, including 635 and 855 nm wavelengths, the oxygenation of biological tissue was evaluated. Finally, the developed LSCI microscope was implemented for the studies of tissue microcirculation. The results indicate that the developed LSCI microscope could be a potential tool for in vivo observation of the tissue microcirculation and quantitative evaluation of hemodynamics in animal experiments.