Fast and sensitive fluorescent probe for ratiometric detection of hydrogen sulfide in mitochondria

A probe Mito-BT was constructed and synthesized by combining triphenylamine and hemicyanine, and can be used for specific ratiometric sensing of H2S. It has a rapid response time of 10 s and preferentially targets to mitochondria. Then the visualization of the change of the mitochondrial H2S concentration upon NaHS incubation by using Mito-BT staining is promising for the practical exploration of H2S-related processes in mitochondria.