Investigation on the 2D-Distribution of Metallic Elements after Hair Dyeing

Long-term use of hair dyes has potential effects on metal content in hair. However, little research dissects the specific distribution and composition variations of the metal after dyeing. In this study, we investigated the morphological change and metallic elements content variation after dyeing. The results showed that the concentration of essential metal elements decreased, among which the Ca, K, and Na decreased sharply even above 50%. As for the heavy metal, the most significant observation is that Pb increased almost by five times after dyeing. Besides, it revealed, using scanning electron microscope coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), that Pb concentrated at the outer layer of the hair. In addition, two-dimensional proton-induced X-ray emission (2D-PIXE) was applied to analyze the distribution of metallic elements along the longitudinal and cross section of the hair. The results showed that Ca and Zn distributed evenly in the hair along the longitudinal and cross section. It is the first time that 2D-PIXE is applied to analyze the metallic distribution in the hair. This method exhibits high sensitivity and can be widely used in the environmental and medical field to analyze the distribution of metallic elements.