The enzymic determination of 2,3-diphosphoglycerate in a crude cell extract (UV method).

Abstract 2,3-Diphosphoglycerate (2,3-DPGA) can be determined in the presence of 3-PGA by a coupled enzymic uv method with the oxidation of NADH as an indicator reaction. This method utilizes the determination of the reaction rate of nonactivated 2,3-DPGA phosphatase activity for purposes of extrapolation. 2,3-DPGA can therefore be determined quantitatively in crude cell extracts such as those obtained from deproteinized blood.