Glycine propionyl l-carnitine attenuates d-Galactosamine induced fulminant hepatic failure in wistar rats.

Abstract Glycine propionyl l -carnitine (GPLC) is a propionyl ester of carnitine that includes an additional glycine component. The present study evaluated hepatoprotective effect of GPLC in d -Galactosamine ( d -GalN) induced fulminant hepatic failure. Rats were intraperitonially administered d -GalN (700 mg/kgBW). GPLC was given as a pre-treatment (35 mg/kgBW/day) for 1 month followed by a single dose of d -GalN on the 31st day. d -GalN administration resulted in increased mortality and serum ALT and AST activities. These increases were significantly attenuated by GPLC. d -GalN treatment increased hepatic lipid peroxidation and a decrease in reduced glutathione content was observed. GPLC pre-treatment significantly decreased lipid peroxidation and augmented the level of GSH. d -GalN increased the circulating level of TNF-α and ATM-Kinase and MAP-Kinase expression. GPLC supplementation inhibited the increase in serum TNF-α and ATM-Kinase and MAP-Kinase expression. d -GalN treatment increased the level of Bax and Caspase-3 m-RNA while as a decline was observed in Bcl2 m-RNA. GPLC prevented the increase in Caspase-3 and Bax m-RNA and at the same time augmented the expression of Bcl2 m-RNA. Our findings suggest that GPLC alleviates d -GalN induced liver injury by strengthening antioxidative defense system and reducing apoptotic signalling pathways.