Genetic Transformation of Trichosanthes kirilowii Maxim. by Ti Plasmid and Formation of Plantlets

Crown galls were induced by direct infection of sterilized seedlings of Trichosanthes kirilowii Maxim. with Agrobacterium tumefaciens C58. Transformation of Ti in C58 was inspected by high_pressure_paper electrophoresis; the detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully. The crown tissues produced plantlets on MS medium and the plantlets were successfully transplanted and grew well.