Study of the Anticarcinogenic Activity of Lactobacillus Bulgaricus in Diethylnitrosamine Induced Cancerogenesis in White Rats

ABSTRACTThe anticarcinogenic activity of living standardized culture of Lactobacillus delbruescii sub. bulgaricus 51-L (LB) was studied on male rats “Whistar”, treated with diethylnitrosamine (DENA). The animals were divided into the following groups: I group—controls; II group—daily LB intake dose of 2 ml/animal with the drinking water; III group—treated with DENA at a dose 130 mg/kg body weight injected intraperitoneally once per week for 8 consecutive weeks; IV group—treated simultaneously with LB and DENA. The animals were monitored during a period of 16 weeks. Their general condition, weight increase, mortality rate and manifestation of tumours were observed. After the observation period the animals were killed and the following parameters were studied: glutathione-S-transferase (G-S-T) activity in liver, macroscopic examination of the animals, histomorphological and histochemical investigation of the suspected ones for tumours and precancerogenic formations as well as liver, kidneys, spleen, lymph n...