Fermentation technique with pichia yeast expressing recombinant protein

Provided is a fermentation technique with a Pichia yeast expressing a recombinant protein. The fermentation technique uses Pichia pastoris as the fungal strain. The method comprises: performing primary seed culturing to reach a fungal concentration of 20±2 g/L, then switching to secondary seed culturing to reach a concentration of 120±2 g/L, then switching to a glycerol culturing phase, wherein the amount of glycerol added for the glycerol culturing phase is 60-70 g/L, then waiting for dissolved oxygen to reach a relatively stable state to initiate a methanol induction phase, and performing induction for 120±8 h to complete the fermentation process.