SYNTHESIS CHARACTERISATION AND BIOLOGICAL EVALUATION OF (3Z, 4Z)-3, 4-BIS (SUBSTITUTED PHENYL BENZYLIDINE)-1-(4-SUBTITUTED PHENYL) PYROLIDINE-2, 5-DIONE

Objective: The present study focuses on synthesis and characterization of new series of bis-chalcone derivatives from cyclic imides and evaluating its antimicrobial activity against a pathogenic microorganism. Methods: The substituted cyclic imides 4-methyl phenyl pyrrolidine 2,5 dione 1 and 4-methoxy phenyl pyrrolidine 2,5 dione 2 were synthesized from succinic acid and primary aromatic amines. These cyclic imides condensed with substituted benzaldehydes in the presence of acetic acid furnished bis-heterocyclic chalcones and characterized by FT-IR, 1 H NMR and also microbial activity was determined using the disc diffusion method. Results: The antimicrobial activity of bis-heterocyclic chalcones was tested against selective pathogens and the zone of inhibition was observed in E. coli, C . albicans and A. niger at 100µg/ml concentration. Conclusion: The compound (3Z,4Z)-3,4-bis-(2-hydroxybenzylidine)-1-p-tolylpyrrolidine-2,5-dione (3b) with methyl and hydroxyl substituent on benzene ring exhibited good antibacterial activity against E. coli and potent antifungal activity against C . albicans and A. niger. Keywords: Succinic acid, Cyclic imides, Pyrrolidine-2,5-dione, Bis-chalcone