Crystallization of the cpn6O/cpn10 complex (‘holo-chaperonin’) from Thermus thermophilus

A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilis has been isolated and crystallized. The crystals have dimensions up to 30 × 200 × 200 μm. Ultra-thin sections of the crystals estimated by electron microscopy showed a rectangular lattice with unit cell parameters of a=17 nm, b=27 nm, γ=90°.