SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions

Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors. PeerJ PrePrints | https://dx.doi.org/10.7287/peerj.preprints.1555v1 | CC-BY 4.0 Open Access | rec: 1 Dec 2015, publ: 1 Dec 2015