Chromosome assignment of a human gene for argininosuccinate synthetase expression in Chinese hamster X human somatic cell hybrids

Abstract The Chinese hamster cell line Don lacks detectable activity of the enzyme argininosuccinate synthetase (ASS). Analysis of somatic cell hybrids formed between Don and a human fibroblast which has ASS activity suggested that ASS expression is a dominant trait which segregates out of those clones which have lost the relevant human gene(s). Forty independent primary hybrid clones were analysed with respect to the correlation of ASS with human chromosomes and linkage markers. This analysis suggested a linkage between a human ASS gene and adenylate kinase-1 on chromosome 9, and this relationship was confirmed in thirty subclones derived from four of the primary hybrid clones.