Expression Systems, mammalian cells

Introduction General Considerations and Strategies for Optimizing Expression Host Cell Types Vector Systems Kozak Sequence Translational Enhancers Selectable/Amplifiable Markers Transfection Methods Growth Media Regulated Expression Dicistronic Vectors Applications of Mammalian Cell Expression Technology Specific Examples of CHO Cell Expression Specific Examples of Myeloma Cell Expression Conclusions Nomenclature Bibliography Keywords: 293 cells; amplification; CHO cells; COS cells; mAbs; regulated expression; selectable markers; stable expression; transient expression