Nuclear matrix proteins differentially expressed in human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line

Objective: To compare the expression of nuclear matrix proteins(NMPs) in benign prostatic hyperplasia(BPH) epithelial cell line BPH-1 versus those in androgen-dependent human prostate cancer cell line LNCap and androgen-independent prostate cancer cell line PC-3.Methods: We isolated NMPs from the BPH-1,LNCap and PC-3 cell lines by 2-dimensional electrophoresis(2-DE),analyzed the differentially expressed proteins by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS),and identified them by peptide mass fingerprint and database searching.Results: We successfully obtained well-resolved reproducible 2-DE patterns of NMPs in human prostate cancer cell lines,identified 12 differentially expressed NMPs including enzymes,regulatory proteins,RNA-binding protein and various other factors,3 up-regulated and 9 down-regulated in prostate cancer cell lines.Conclusion: There are obvious differences in the expressions of NMPs between human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.