ISSR Analysis on Genetic Diversity of Plantago asiatica L.Germplasm Resource

[Objective] To study the genetic diversity of Plantago asiatica L. germplasm resource from seven provinces such as Jiangxi Province, Hunan Province and Hubei Province. [Method] ISSR technology was used to analyze the genetic diversity and genetic relationship of 28 copies of Plantago asiatica L. samples. [Result] Sixteen primers that were selected at total forty primers were used to analyze the genetic diversity. 131 strips were amplified, among which 107 strips had polymorphism, accounting for 81.7% of the total amount. ISSR data showed that the scale of diversity point (P) was 75.3%, observed number of alleles was 0.071 3, effective number of alleles was 0.299 0, Nei's gene diversity (H) was 0.360 1, Shannon's Information index (I) was 0.535 4. [Conclusion] The genetic diversity of Plantago asiatica L. germplasm resource had great difference at geography. The wild varieties and cultivated varieties had great difference at gene type.