Analysis of estrogen levels in the urine of pregnant women using liquid chromatography

: The gas chromatography estimation using CHROM IV device enables a simultaneous investigation of oestrone, oestradiol and oestriol in urine. It is possible to use sorption on a solid sorbent PRESEP C 18 TESSEK for steroid extraction from biological material which shortens and simplifies the investigation method. The oestriol results gained by the described technique are compared with the values of total oestrogens estimated by the classical fluorometric method after Ittrich. The linear regression analysis gives the following result: Y = 0.7637x-2.7531, r = 0.9431, p less than 0.001 and confirms the correlation of both methods.