Effects of combined florfenicol and chlorogenic acid to treat acute hepatopancreatic necrosis disease in Litopenaeus vannamei caused by Vibrio parahaemolyticus

Abstract We evaluated the survival, disease resistance, and immunity of Litopenaeus vannamei infected with Vibrio parahaemolyticus causing acute hepatopancreatic necrosis syndrome (VPAHPND), and treated with florfenicol (FFC) and chlorogenic acid (CGA), alone or in combination. Following VPAHPND challenge, the shrimps were fed a drug-free diet (control), or FFC (15 mg·kg−1), CGA (200 mg·kg−1), low-dose drug combination (100 mg·kg−1 CGA + 7.5 mg·kg−1 FFC), moderate-dose drug combination (200 mg·kg−1 CGA + 15 mg·kg−1 FFC), and a high-dose drug combination (400 mg·kg−1 CGA + 30 mg·kg−1 FFC) diets for 5 days. Compared with either drug alone, the shrimps in the FFC and CGA combination groups showed significantly lower cumulative mortality over 5 days after infection (p