Developing bcl-x_l transgenic mice by microinjection

It was attempted to develop bcl x l transgenic mouse and produce its lineages for studying ischemic cerebrovascular diseases,bcl x l gene was introduced into mice by microinjection .The presence of bcl x l and its expression were confirmed by PCR,Southern blot,mRNA inspection,Western blot in the founders.In our experiments, bcl x l gene was injected into pronucleus of 1 654 zygotes and 1 448 injected eggs were implanted into 49 foster mothers, 7 of which were pregnant and bore 13 offsprings. Among the babies, 4 offsprings were integrated with the bcl x l gene and expressed it. Totally, implanted zygotes survival rate (83%), zygotes total survival rate (0.78%), recipient mouse pregnance rate ( 14.3% ), integration rate (30.7%), expression rate(100%) ,total efficiency(0.24%)were obtained respectively. Bcl x l transgenic mice were obtained, 4 in number, but the transgenic efficiency was very low and the expression was unstable. The model of cerebral vascular disease will be made after acquiring transgenic lineages.