Análisis morfométrico del efecto del láser infrarrojo sobre hepatocitos de rata

El laser infrarrojo emitido por el diodo Arsenurio de Galio (904 nm) proporciona terapia a lesiones articulares por su accion analgesica, cicatrizante y antiinflamatoria, promoviendo a nivel celular sintesis de ATP mitocondrial, modulacion de canales de calcio, activando el proceso mitotico e incremento en la sintesis de DNA y de proteinas. Para determinar las dosis que estimulen componentes celulares involucrados en sintesis proteica, del higado de ratas fueron tomadas muestras de tejido normal e irradiado mediante laser infrarrojo con 1, 2, 4, 8 y 16 Joules/cm2 durante 15 dias consecutivos. Fueron tratadas para microscopia electronica de transmision y se obtuvieron micrografias con aumentos de 10.000 X. Se realizaron estudios morfometricos, cuantificandose las fracciones volumetricas de nucleos, citoplasma, reticulo endoplasmatico rugoso (RER), inclusiones de glicogeno, nucleolos, eucromatina y heterocromatina, relacion nucleo- citoplasmatica y las areas celulares y nucleares. Los resultados del presente estudio que compara hepatocitos normales e irradiados, indican que existen diferencias significativas en todos los parametros evaluados. Se concluye que los hepatocitos estimulados alteran su morfologia y por ende sus componentes celulares, modificando la funcion celular determinandose con exactitud la dosis de estimulacion infrarroja donde estas celulas presentan un mayor desarrollo de su maquinaria citoplasmatica involucrada en sintesis de proteinas. The infrared lasser emitted by the Gallium Arsenide diode provides an adequate therapy for articular lessions due to their healing, analgesic, and anti-inflammatory powers. It also promotes at cellular level mitochondrial ATP synthesis, modulates Calcium channels and activate mitotic processes by increasing DNA and protein synthesis. To determine the effective doses which stimulates rat liver protein synthesis, several samples from normal and irradiated tissues to intensities of 1, 2, 4, 8, and 16 Joules/cm2 by 15 consecutive days were taken. These samples were later prepared and observed under transmission E.M. (10000X) and analyzed by morphometric studies, where volume and organelle distribution, such as nucleus, cytoplasm, endoplasmic reticulum, glycogen inclussions, nucleolus, eu and heterochromatin were accounted, together at nuclear-cytoplasmic relationships and the cellular and nuclear areas. Under comparison normal and irradiated hepatocytes presented a significative difference in all evaluated parameters. It can be concluded that at certain specific level of infrared irradiation, hepatocytes alter their morphology by modifyng those cellular components involved in protein sintesis.