Use of corticosteroid antibodies for the study of corticosteroid biosynthesis in vitro

Abstract 1. 1. Radioimmunochromatographic analysis of adrenal incubates is a useful method for the characterization of uncommon and unidentified steroid fractions. In incubates of normal adrenals at least seven uncommon fractions were separated. 2. 2. Uncommon and unidentified steroids may react also with antibodies of apparently high specificity. This observation may have practical consequences for in vivo studies. 3. 3. Under pathophysiological conditions such as adrenal regeneration hypertension steroids may be produced by adrenocortical tissue which are not detectable in incubates of normal adrenals.