Effect of Ethanol Extract of Venenum Bufonis on Biofilm Formation of Staphylococcus aureus

Ethanol extract of Venenum Bufonis has shown many valuable bioactivities, but little is known about its effect on biofilm formation of Staphylococcus aureus. In this study, biofilm formation of S. aureus NCTC8325 with or without ethanol extract of Venenum Bufonis was tested using microtitre plate assay and Confocal Laser Scanning Microscope (CLSM) system. Results show that the biofilm formation of S. aureus with ethanol extract of Venenum Bufonis was significantly lower than that of the control group without ethanol extract of Venenum Bufonis. Meanwhile, the reduction degree was correlated to the concentration of ethanol extract of Venenum Bufonis positively. With CLSM system we can observe that looser and less biomass of the biofilm structure of the experimental group appeared than that of the control group. These results suggested that ethanol extract of Venenum Bufonis has inhibitory effect on biofilm formation of S. aureus.