Genomic analysis of one Multidrug-resistant Klebsiella pneumoniae ST11 strain recovered from Barbary Deer (Cervus elaphus barbarus) in the Akfadou forest, Algeria.

Abstract Background The emergence and worldwide spread of carbapenemase-producing Enterobacterales (CPE) is of great concern to public health. The present study aimed to screen for the presence of CPE isolates from Barbary Deer in Akfadou forest, Bejaia (Algeria). Methods Fecal samples (n = 39) were obtained from Barbary Deer animals in Akfadou forest, Bejaia (Algeria) between March and June 2018. Whole-genome sequencing (WGS) was performed to characterize one strain of Klebsiella pneumoniae. Data analysis was performed using online tools. Results Thirteen carbapenem-resistant K. pneumoniae isolates were obtained. They showed an identical antibiotic resistance pattern and were susceptible to colistin and fosfomycin. WGS analysis revealed the complete resistome of the K. pneumoniae strain CF21 including blaNDM-1, blaCTX-M-15, blaSHV-182, blaDHA-1, blaOXA-1, aac(3)-IIa, aac(3)-IId, aac(6’)Ib-cr, rmtC, sul1, qnrB9, fosA, tetA, dfrA14, catA2,catB3, and mphA. The multilocus sequence typing (MLST) analysis assigned this strain to the international clone ST11. Plasmid analysis showed that this K. pneumoniae strain possesses five different plasmids including IncA/C2, IncFIA (HI1), IncFIB (K), IncFII (K), and ColRNAI. Conclusion This study reports one multidrug-resistant K. pneumoniae strain recovered from Barbary Deer in Algeria and confirms that wild animals could serve as a reservoir of antibiotic resistance genes.