Analysis of heading time genotype for a rice male sterile line Zhenshan 97A

Zhenshan 97A, a rice male sterile, were applied widely si nce the release of hybrid rice in 1973 in China, but the genotype of heading tim e in this sterile line was still unknown. This definitely limited the further us e of this sterile line in breeding practice and re-production of hybrid seeds. To solve this problem, we analyzed the segregation pattern of phenotype of headi ng time in progenies from crosses between Zhenshan 97A and four tester cultivars , Akihikari (e 1e 1e 2e 2e 3e 3 Se-1eSe-1e), Koshihikari (E 1E 1 E 2E 2e 3e 3 Se-1eSe-1e), Nipponbare (E 1E 1e 2e 2e 3e 3Se-1 Se-1) and Hinohikari (E 1E 1E 2E 2e 3e 3S e-1Se-1),whose genotypes of heading time were already known. The results showed that the genotype of heading time in Zhenshan 97A was e 1e 1e 2 e 2E 3E 3Se-1Se-1 and it also carri ed a recessive inhibitor i-Se-1 for phtoperiod-sensitivity. Mea nwhile, a ma jor photoperiod-sensitive dominant genes Se-1 and other modifie d photoperio d-sensitive genes: i-Se-1, E 3,Hd3 (En-Se-1), Hd5 and Hd6, were ident ified in Zhenshan 97A by crossing with QTL nearly isogenic lines: NIL (Hd1),NIL (Hd2),NIL(Hd3),NIL( Hd5) and NIL(Hd6).