Effects of dietary mannan oligosaccharide supplementation on growth performance, antioxidant capacity, non-specific immunity and immune-related gene expression of juvenile hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Abstract The present study was conducted to investigate the effects of dietary mannan oligosaccharide (MOS) supplementation on growth performance, antioxidant capacity, non-specific immunity and immune-related gene expression in juvenile hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). A basal diet supplemented with MOS (0, 0.3%, 0.6%, 1.0% and 2.0%) was fed to juvenile hybrid grouper for 9 weeks. Results showed that there were no significant differences in feed intake, hepatosomatic index, spleen index or survival among all groups (P > .05). However, antioxidant analyses showed that hybrid grouper fed with MOS supplementation had significantly enhanced total antioxidant capacity (T-AOC) and superoxide dismutase (SOD). Non-specific immunity evaluation showed that hybrid grouper fed with MOS supplementation had significantly enhanced respiratory burst activity (RB), acid phosphatase activity (ACP) and lysozyme activity (LZM) (P