The Significance of DNA Content Analysis by Flow Cytometry in Pathological Assessment of Otolaryngocarcinoma

Objective: To study the significance of DNA content analysis by flow cytometry in pathologic assessment of otolaryngocarcinoma.Methods: DNA ploidy was analyzed with flow cytometry and pathological examination was undertaken to 106 specimens including 18 cases of inflammatory tissue(IT group),32 cases of atypical hyperplasia(AH group),and 56 cases of carcinoma(CA group,21 of nasopharyngeal carcinoma,31 of laryngocarcinoma,and 4 of lower-pharyngeal carcinoma).Results: 1) 2 cases of heteroploid were found in IT group(2/18,11.11%),30 in AH group(30/32,93.75%),and 47 in CA group(47/56,83.92%);2) Heteroploid level of AH group differed from that of IT group(P0.01),while no significant difference of S cell was found between the 2 groups(P0.01);3) Heteroploid level and S cell level of CA group were different from those of IT group(P0.01);4) The difference between Heteroploid levels of AH group and CA group was not significant(P0.01),while the difference of S cell levels between them was obvious(P0.01).Conclusion: DNA content analysis is helpful in fast diagnosis of otolaryngocarcinoma.