Optimiztion of Extraction Technology of Apocynum venetum in Jieduyin Pills

Objective: To optimize extraction technology of Apocynum venetum in Jieduyin pills.Method: With the content of hyperoside and analgesic efficacy reaction as indexes,extraction solvent was selected by single-factor test;With the content of hyperoside and dry extract yield as comprehensive index,effect of the amount of solvent,extraction time and frequencies on extraction technology was investigated by orthogonal test.the content of hyperoside was determined by HPLC.Result: Optimum extraction technology was as following: extracted 3 times with 12 times the amount of 50% ethanol,1 h each time.Conclusion: This optimized extraction technology was stable and effective,which could lay foundation for further study of Jieduyin pills.