Total Flavonoids Extraction from Radix Scutellariae and Its Purification by High-speed Counter-current Chromatography

Objective Total flavonoids extraction process from Radix Scutellariae by ethanol reflux was investigated,and high-speed counter-current chromatography(HSCCC)was applied for the separation and purification of flavonoids.Methods The optimal extraction condition for flavonoids was investigated with orthogonal test design,and high-puri-ty target of flavonoids was obtained by HSCCC.Results The optimal condition of extraction was: extraction with 60 % ethanol for two times,the solvent being 12,10 times as much as the herbal materiall,and extraction time being 2 hours,1 hour.Under the above condition,content of total flavonoids in the crude extract was up to 15.8 %.The ex-periment of HSCCC was performed with a two-phase solvent system composed of chloroform-methanol-water-acetic acid(4 ∶ 3 ∶ 3 ∶ 0.1,V/V).When the injection volume of crude extract was 400 mg,45 mg total flavonoids was ob-tained with a purity of 90.2 %.Conclusion High-purity flavonoids from Radix Scutellariae is obtained after ethanol reflux extraction and purification by HSCCC.