Effects of dialyzed membrane on TGF-β1 and MCP-1 plasma levels in hemodialysis patients

Objective To investigate effects of different dialyzed membrane on transforming growth factor(TGF-β1 ) and monocyte chemoattractant protein(MCP-1 ) plasma levels in maintenance hemodialysis (MHD) patients. Methods The plasma concentrations of TGF-β1 and MCP-1 were measured by ELISA assay in well-matched uremic patients undergoing maintenance hemodialysis with either cuprophane (CU, n = 27 ) or polysulfone (PSU, n = 25 ). Results Compared with controls (50. 2 ± 5. 7 ng/ml), the patients on CU(82. 9 ± 9. 8 ng/ml) and PSU (65. 5 ± 6. 5 ng/ml)membranes dialysis had significantly higher mean TGF-β1 concentration (P 0. 01, P 0. 05). However, the patients on PSU membrane dialysis had significantly lower mean TGF-β1 concentrations in contrast with CU membrane dialysis. MCP-1 plasma levels were also higher in patients dialyzed with PSU(72. 2 ± 5. 3 pg/ml) than in controls(56. 4 ± 7. 8). In contrast to TGF-β1, the increase was greater in PSU-treated patients than in CU-theated patients. In addition, negative correlation was observed between the plasma levels of TGF-β1 and MCP-1 in patients dialyzed with CU or PSU. Conclusions MHD is associated with the increase of plasma levels of TGF-β1 and MCP-1. Moreover, the type of dialysis membrane has different effects on plasma levels of TGF-β1 and MCP- 1.