Analysis of the mitochondria gene NADH dehydrogenase subunit 1 and development of PCR detection in Echinococcus multilocularis

Objective To analyze the whole length of the mitochondria gene ND1 in Echinococcus multilocularis and to develop a PCR method for detecting and identifying E.multilocularis infections in human and animals.Methods According to the whole length of the DNA in E.multilocularis,primers were designed to amplify the mitochondria gene ND1.Results The mitochondria gene ND1 in E.multilocularis we sequenced had 98.8％ homology with E.multilocularis,86.2％ with E.grarulosus,84.6％ with E.oligarthrus,and 87.0％ with E.vogeli from abroad.Conclusion There were distinctive variations between E.multilocularis and other Echinococcus spp.PCR technique is a fast method for E.multilocularis detection. Key words: Echinococcus multilocularis; Mitochondria gene ND1; PCR detection