Evaluation of antibacterial properties on polysulfone composite membranes using synthesized biogenic silver nanoparticles with Ulva compressa (L.) Kütz. and Cladophora glomerata (L.) Kütz. extracts

Abstract Polysulfone (PS) composite membrane using green synthesized biogenic silver nanoparticles (Ag-NPs) with Ulva compressa (L.) Kutz. and Cladophora glomerata (L.) Kutz. extract were prepared by spin coating technique and are tested for antimicrobial activity using a direct contact test for the first time. Initially green synthesis of Ag-NPs was accomplished utilizing green macro algae i.e. U. compressa (L.) Kutz. and C. glomerata (L.) Kutz. by the reduction of AgNO 3 . The Ag-NPs/PS composite membranes from both algae revealed outstanding antimicrobial activity against all bacteria i.e. K. pneumonia , P. aeruginasa, E. coli , E. faecium and S. aureus . Bacterial growth was monitored for 17 h with a temperature controlled microplate spectrophotometer. The kinetics of the outgrowth in each well were recorded continuously at 630 nm every 60 min. Thus present work remarkably offers a feasible, cheap and efficient alternative for making Ag-NPs and their utilization as antimicrobial agent on the PS composite membrane.