Retinoic acid receptor β2 and neurite outgrowth in the adult mouse spinal cord in vitro
2002
Retinoic acid, acting through the nuclear retinoic acid receptor β2
(RARβ2), stimulates neurite outgrowth from peripheral nervous system
tissue that has the capacity to regenerate neurites, namely, embryonic and
adult dorsal root ganglia. Similarly, in central nervous system tissue that
can regenerate, namely, embryonic mouse spinal cord, retinoic acid also
stimulates neurite outgrowth and RARβ2 is upregulated. By
contrast, in the adult mouse spinal cord, which cannot regenerate, no such
upregulation of RARβ2 by retinoic acid is observed and no
neurites are extended in vitro. To test our hypothesis that the upregulation
of RARβ2 is crucial to neurite regeneration, we have transduced adult
mouse or rat spinal cord in vitro with a minimal equine infectious anaemia
virus vector expressing RARβ2 . After transduction, prolific
neurite outgrowth occurs. Outgrowth does not occur when the cord is transduced
with a different isoform of RARβ nor does it occur following
treatment with nerve growth factor. These data demonstrate that
RARβ2 is involved in neurite outgrowth, at least in vitro, and
that this gene may in the future be of some therapeutic use.
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