Two-stage screening of combinatorial peptide libraries. Application to bovine serum albumin ligand selection
2015
Short peptides are excellent ligands for affinity chromatrography. Divide-couple- recombine method allows obtaining a peptide library with all possible combinations of the amino acids in the form of "one bead-one peptide". It was designed a two-stage library screening method for peptide ligands selection in this work. The library screening was performed with the target protein (bovine seroalbumin, BSA) coupled to Texas Red. Fluorescent beads were automatically
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