Vascular Responses to Extractable Fractions of Ilex paraguariensis in Rats Fed Standard and High-Cholesterol Diets

The authors investigated the vasorelaxant properties of the aqueous (Aq-EF) and acid n-butanolic (acn-BuOH) extractable fractions from Ilex paraguariensis leaves. Perfusion pressure was evaluated using isolated and perfused mesenteric arterial beds (MABs) from rats fed hypercholesterolemic and standard diets. Extract-induced vasorelaxation in the presence and absence of various inhibitors was examined following precontraction of the MABs with methoxamine (30 μM) solution. In hypercholesterolemic-diet rats, relaxation in intact MABs was significantly decreased with ac-n-BuOH-EF bolus (300, 600, 900 μg) in comparison to those in standard-diet rats. After the endothelium was stripped from the MABs, the vascular responses to ac-n-BuOH-EF and 900 μg bolus of Aq-EF were significantly changed. Treatment of the MABs with an inhibitor of nitric oxide synthase, NG-nitro-L-arginine methylester hydrochloride (L-NAME, 10 mM), did not change either ac-n-BuOH-EF- or Aq-EF-induced vasodilation except for the 900 g bolus ...