Basic research of beta1-adrenergic receptor gene methylation on the different expression and correlation with blood pressure in spontaneously hypertensive rats

Objective: To investigate the changes of isomers gene expression about α subunit of sodium pump in cellular membrane during the senescence of human umbilical vein endothelial cells (HUVEC). Methods: A cellular ageing model was built according to extracting, appraising and culturing of the original generation HUVEC. And then it is divided into different groups by giving d-galactose and fragment of extra-cellular segment of sodium pump α subunit (RES ramification) in different PD (Population). The cellular ageing was determined by the intervention of cellular morphology and the dye of senescence associated betad-galactosidase. In addition, the methods of immune cell fluorescence staining, real-time PCR and western blotting were used to analyze the gene expression change of sodium pump α-subunit both in mRNA and protein levels. Results: The expression of sodium pump α1, α2 and α3 unit were all decreased with the increase of the PD in not only mRNA and but also protein levels (Pb0.01); In the same PD, the expression of sodium pump α2 and α3 unit in control group was higher than that in D-galactose group but lower than RES ramification group (Pb0.05). However, there are no significant differences of α1 subunit of sodium pump expression in each group (PN0.05). Conclusions: The unbalanced expression of sodium pump α subunit in cellular membrane may be one of molecular mechanisms of HUVEC aging. It is that the RES ramification plays an important role in defending against the senescence of HUVEC according to recovering the activity of sodium pump α2 and α3 unit partially.