Anti-HSV-1 Activity of the Extract from Fructus Toosendan In-vitro

Objective To investigate the in-vitro effect of the extract from Fructus toosendan on counteracting the activity of herpes simplex virus type 1(HSV-1). Methods By using Vero cell as the host cells and with acyclovir as the positive control,anti-HSV-1 activity of the extract was observed and determined with cytopathic effect (CPE) assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects:virucidal assay,attachment assay and penetration assay. Results The extract had conspicuous anti-HSV-1 activity,with an IC50 value of 84.28 μg/mL and a therapeutic index (TI) value of 18. The extract could inactivate virus directly,but had no influence on virus attachment and virus penetration. Conclusion The extract from Fructus toosendan has conspicuous anti-HSV-1 activity by inactivating virus directly.