The Prevention and Therapeutic Effects of Hsp70-Antigen Peptide Complexes on B16 Tumor Pulmonary Metastasis in Mice

Objective: To explore the preventive and therapeutic effects of Hsp70-antigen peptide complexes (HACs) on pulmonary metastasis of malanoma B16 cells in mice. Methods: Antigen peptide mixtures were prepared from massive tumors and metastatic foci respctively, and bound with Hsp70 in vitro. The HACs were used to mice to prevent the formation of micrometastatic foci after the injection of B16 cells through tail vein, or treat the existent micrometastatic foci in lung The number of metastatic foci was counted and the cytotoxicity of splenocytes to B16 cells was determined. Results: After immunization with HACs, the number of metastatic foci in mice decreased significantly (P 0. 01) , and the cytotoxicity of splenocytes to B16 cells was signficantly enhanced (P 0. 01) . The immuniation with HACS can both prevent the formation of metastatic foci and freat the existent metastatic foci. The immunization with petide mixture from massive tumros produced better therapeutic effect ( P 0. 01) , and induced stronger citotoxicity of splenocytes to B16 cells (0. 01 P0. 05) , than peptides from metastatic foci. Conclusions: HACs can induce significant preventive and therapeutic effect on tumor metastasis, and peptides from massive tumors were more effective than those from metastatic foci.