Efficient Production of Biodiesel from Rapeseed Oil Deodorizer Distillate: One-Pot Esterification and Transesterfication with Compound Lipases

A novel concept and efficient method for producing biodiesel from rapeseed oil deodorizer distillate (RODD) was developed by using compound lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system. Compound lipases Novozyme435 and immobilized Pseudomonas cepacia G63 at 1:1(wt/wt) gave FAME about 96% yield under optimal conditions, being better than that with single lipase or other compound lipases. Besides, the loss rate of vitamin E was 18% much lower than that of using chemical catalyst, which shows that the compound lipases Novozyme435 and immobilized P. cepacia G63 can be ideal catalysts for biodiesel production from rapeseed oil deodorizer distillate.