ВЫЯВЛЕНИЕ ПЕЙЕРОВЫХ БЛЯШЕК МЫШЕЙ ПУТЕМ ЗАПОЛНЕНИЯ КИШЕЧНИКА КОНТРАСТНОЙ МАССОЙ

At the Institute of Experimental Veterinary Science of Siberia and the Far East (Krasnoobsk, Novosibirsk Region) was developed a method for identifying grouped lymphoid nodules (Peyer’s patches) in the small intestine of mice by filling it with the contrast mass. It is shown that the Peyer’s patches are well seen against its background, with their contours outlined more sharply. For contrasting the patches, 1.2 ml of ink was intragastrically injected to the mice weighing 20-22 g 75 minutes before slaughtering. With that, one day before using the method the feeding of animals was stopped, the access to water being not limited. It has been established that when the intestine is filled with ink due to the peristaltic movements (in contrast to its filling under pressure in slaughtered mice), the distension of the intestine wall and distortion of the grouped nodule size don’t occur. The dose of 0.01 mg of atropine sulfate was added to ink to ensure the uniform ink distribution in the intestine. The use of the antispasmodic agent allowed filling the small intestine with the contrast mass without empty spaces that is necessary for contrasting all the Peyer’s patches. It is shown that the injection of ink and atropine in the mentioned doses doesn’t exert negative influence on the general condition and behavior of the animals. The noticeable effect of atropine on the patch size wasn’t detected. The comparison of the results from counting and measuring the patches in the groups with contrasting and without it was made. It has been found that the proposed method allows increasing the number of the patches detected by 45%, thus the index of the average area of the patch increases by 21% (p < 0.05). The histological examination of the small intestine was carried out after contrasting. Any negative impact on the microscopic structure of the intestinal mucosa including the columnar epithelial cells of apexes of the villi and their striated border wasn’t revealed. The soot, which is a part of ink, didn’t interfere with the production of the histological sections.