Induced Herbicide Resistance in Certain Food Legumes Using In Vitro Techniques

Recently new approaches were developed to produce tissue and cell culture techniques capable of regeneration in to fertile plants in recalcitrant Legume crops by via either Organogenesis or Embryogenesis. The suspension culture system facilitates an experimental approach with a large variety of objectives for crop modification cellular selection and transformation. The importance of plant cell and tissue in applied and fundamental research has out lined by many workers. Though Legumes have been extensively used in plant cell and tissue culture systems regeneration of whole plants from in vitro experiments not amicable like other systems. The growth of excised plant tissues on defined medium should provide a relatively precise measurement of the effect of growth regulating substances as compared with the evalution of their influence on whole plant. This approach is tested in the food Legumes by comparing auxin and cytokinin requirements on callus tissue derived from different genotypes. The objective was to identify normal and plant types with distinct auxin and cytokinin response to be used in studies of genetic regulation of hormonal function and metabolism.