Methodological approach to the isolation of functionally active proteins from the tissues of marine hydrobionts: an example of Adamussium colbecki

Enzymes from cold-adapted organismshave significant application potential. Because of their unique properties they have been found to be useful in various industries. Despite indisputable practical interest, cold active enzymes also represent a valuable model for fundamental research into protein folding and catalysis. Many investigators have focused their attention on marine hydrobionts, which are growing in importance as a promisingsource of enzymes.The nature of the source not only determines the availability and the cost of biomolecules of interest but also determines the choice of method for their extraction. A simple and convenient methodological approach of two-stageextraction of proteins has been tested on the Antarctic marine hydrobiont— Adamussium colbecki . This method extractsenough effective protein directlyfrom primary raw materials, as well as when using leftover crude precipitates. The electrophoretic pattern of proteins showed the presence of molecules in a wide rangeof molecular weights in the samples of A. colbecki after the first and the second stage of extraction. The general proteolytic activity in the first and the second extractswere examined using a zymogram technique. Our experiments revealedthat the second extract of A. colbecki contained thermo stable protease exhibitinga molecular weight of 95 kDa in a gelatin zymogram.Further biochemical assays, using different substrates, were conducted to partially identifythe types of hydrolases present in the first and the second extracts.Our results revealed the presence of enzymes with collagenolytic and some amylolyticactivities preserved in the second extracts.But no esterase or amidase trypsin-like activities were found in the second extract, in contrast to the first extract where this type of activitywas significant. Citation: Raksha N, Gladun D, Savchuk O, et al. Methodological approach to the isolation of functionally active proteins from the tissues of marine hydrobionts: an example of Adamussium colbecki. Adv Polar Sci, 2015, 26: 299-304, doi: 10.13679/j.advps.2015.4.00299