cimp1, A Novel Astacin Family Metalloproteinase Gene from East African Cichlids, Is Differentially Expressed Between Species During Growth

Lake Victoria cichlid fishes are excellent examples of explosive adaptive radiation. Although Lake Victoria cichlids are believed to have arisen during a short period (∼14.000 years), they have various species-specific phenotypes. One important phenotype that distinguishes each species is the shape of the jaw, which has diverged to adapt to the wide variety of trophic habitats present in the lake. Here we demonstrate a new approach to investigate the diversification of cichlid jaw morphology at the genetic level by examining differentially expressed genes. We used a DNA chip to compare gene expression levels between closely related cichlid fishes. This analysis indicated that the expression of some genes differed in the larvae of two cichlid species. One such clone encodes a new astacin family metalloproteinase. The expression level of the isolated gene, named cimp1, was analyzed in more detail by real-time quantitative reverse transcription-polymerase chain reaction. A significant difference in cimpi expression was observed between two Haplochromis cichlid species during development. Using in situ hybridization, we found that this gene is expressed only in head and gill epithelia. Biochemical analysis showed that cichlid metalloproteinase 1 (CiMPI) has proteolytic activity, a common attribute of all astacin family proteins. Because some astacin family proteins contribute to morphogenesis in animals, CiMPI is expected to participate in species-specific head morphogenesis in cichlids. This is the first study to demonstrate that differentially expressed genes among cichlids can be identified using a DNA chip.