Purification of CMP-N-acetylneuraminic acid synthetase from bovine anterior pituitary glands

Justine Vionnet, Nelydia Concepcion1, Thomas Warner2, Gerardo Zapata2, John Hanover3 and Willie F.Vann4 Laboratory of Bacterial Toxins and 1Laboratory of Bacterial Polysaccharides, Division of Bacterial Products, OVRR, CBER, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA, 2Genentech, Inc., 460 Point San Bruno, South San Francisco, CA 94080, USA and 3Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892–0850, USA