Method for determining minim proteins based on magnetic pearl and nano gold probe

The invention relates to a high-sensitivity trace of protein measuring method based on bead and nm metal probe, comprising: labelling the bead using monoclonal antibody of protein to measure; labelling the polyclonal antibody of protein to measure on nm metal probe and at the same time labelling a DNA probe with biotin label on the nm metal probe; labelling horseradish peroxidase on the DNA probe of nm metal probe by biotin-streptavidin reaction to form nm metal probe; mixing the labelled bead and nm metal probe and protein sample and incubating for a period of time at 37 degree; and then washing the nm metal probes which do not react; developing using TMB development system, therefore the protein can be quantificationally detected. The detection time can be reduced to 1-1.5 hours and the sensitivity can be pg/ml.