Isolation and screening of indigenous bacteria from phosphogypsum-contaminated soils for their potential in promoting plant growth and trace elements mobilization

Abstract Bacteria isolated from soils in the vicinity of phosphogypsum (PG) stockpiles were studied for their potential use in bioaugmentation-assisted phytoextraction. Quick, miniaturized biochemical tests were performed in the presence of metal trace elements (MTE), including rare earth elements (Cd, Sr, Ce, La, Nd and Y), corresponding to their bioavailable concentrations in PG. The intention herein was to assess the capacity of bacteria to: i) grow in PG; ii) produce indole acetic acid and ACC deaminase to promote plant growth and reduce stress; and iii) produce siderophores, including pyoverdine, to mobilize MTE. Results showed that even at maximum PG concentration (10 g/L and pH 3.40), 7 out of 32 isolates were able to grow. The biochemical tests showed differences in the presence or absence of MTE. The presence of MTE seems to promote the production of IAA by a factor of 3.25. On the contrary, it inhibits ACC deaminase and siderophore production, including pyoverdine. According to a scoring method applied, the two most efficient isolates exhibiting maximum metabolite production were identified as Bacillus sp.