A method for qualitative and quantitative detection of chemical substances comparative
2002
Disclosed is a method for qualitative and quantitative comparative detection of chemical substances. At least one type of chemical substances and at least one type of marker/receptor unit is provided. The marker/ receptor units respectively consist of a known marker and a receptor unit joined thereto. The receptor unit can bind a chemical substance. The marker component of the marker/receptor units is a desoxyribo nucleic acid oligomer, a peptide nucleic acid oligomer or a nucleic acid oligomer with a structurally analogous backbone. The chemical substances and the marker/receptor units are brought into contact with each other, resulting in the formation of complexes of at least one marker/receptor unit and a chemical substance. The complexes of the marker/receptor unit and a chemical substance are subsequently separated from the non-bound marker/receptor units and one or more chemical bonds of complexes of a marker/receptor unit and a chemical substance are cleaved in such a way that the marker component is separated from the complex of the marker/receptor unit and the chemical substance. The separated marker components are washed out and detected by an appropriate method. According to the inventive method, at least one type of chemical substance is provided which is of the same type of chemical substance as the chemical substances provided above or which is different from said chemical substances. The same type and amount of marker/receptor units as those previously mentioned are also provided. The latter chemical substances and the latter marker/receptor units are brought into contact with each other, resulting in the formation of complexes of at least one marker/receptor unit and a chemical substance. The non-bound marker/receptor units are separated. Subsequently, one or more chemical bonds of complexes of a marker/receptor unit and a chemical substance are cleaved in such a way that the marker component of the marker/receptor unit is separated. The separated marker components are washed out and detected by means of an appropriate method. Finally, the results of the two analyses of the marker components thus obtained are compared.
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