High-speed live-cell super-resolution microscopy with stochastically switching fluorophores

Fang Huang,Tobias M. P. Hartwich,Felix Rivera-Molina,Yu Lin,Jordan R. Myers,Jane J. Long,Michael W. Davidson,Derek Toomre,Joerg Bewersdorf

High-speed live-cell super-resolution microscopy with stochastically switching fluorophores

2013

Fang Huang
Tobias M. P. Hartwich
Felix Rivera-Molina
Yu Lin
Jordan R. Myers
Jane J. Long
Michael W. Davidson
Derek Toomre
Joerg Bewersdorf

Super-resolution microscopy with stochastically blinking fluorophores (FPALM/PALM/STORM, etc.) achieves ∼25 nm resolution by localizing molecules in thousands of camera frames. Here I present a solution to speed up recording dramatically, enabling nanoscopy at video rate.

Keywords:

Optical microscope
Light sheet fluorescence microscopy
Photoactivated localization microscopy
Fluorescence microscope
Superresolution
Physics
Super-resolution microscopy
Microscopy
Optics
Image resolution
video rate
Optoelectronics

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